The problem of the psychological well-being of women employed in the educational field currently still has a fairly wide range of unexplored issues. In this paper, well-being is considered in connection with the components of professional satisfaction and motivation, taking into account gender and age aspects. The study involved 140 people, including 103 women aged 20 to 75 years. The study was carried out using theoretical analysis of scientific literature, the Method for diagnosing the subjective well-being of a person (Shamionov & Beskova), Motivation Sources Inventory (version of Sidorenko), Job Satisfaction Survey (P. Spektor), Maslach Burnout Inventory (version by Vodopyanova & Starchenkova), Izard's Differential Emotions Scale adapted by Leonov and Kapitsa, test “Personal differential” (adapted at the Bekhterev Research Institute). Statistical methods: Shapiro-Wilk test, non-parametric Kruskal-Wallis test, pairwise comparison using the Dunn method, Spearman's rank correlation coefficient, Cohen's q and z- test for comparing two sample correlation coefficients. As a result, differences between different age groups of women were revealed in terms of professional stress, well-being and burnout, as well as differences for women and men in relationship between the psychological aspects of their professional activities and personality traits, emotions and well-being.